Morphological characteristics and ISSR markers, a tool for studying the genetic diversity of tea plant in Iran

Document Type : Research

Authors

1 Tea Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Lahijan, Iran

2 1- Tea Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Lahijan, Iran;

3 Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

Abstract

Tea (Camellia sinensis (L.) O. Kuntze) is one of the most important products in the northern of Iran, many of them are endangered, so having information about their genetics to design breeding programs for special purposes is a great help. In this study, morphological and ISSR markers were used to evaluate the genetic diversity of 28 tea plants from east, center and west of tea culturing region. The use of 21 morphological traits showed narrow range of similarity between the studied tea samplesThe samples were divided into three groups at a difference of 0.86, the main group formed was the second group (B), which contained 57% of the samples. Application of 7 ISSR primers produced 60 bands, of which 52 bands showed polymorphism. The maximum and minimum polymorphic information content (PIC) for all primers were 0.49 and 0.44, respectively. Based on ISSR data, the similarity range was in the range of 0.31 to 0.87. In cluster analysis, the samples were divided into two groups at the similarity level of 0.54, with the second group being the largest group with a coverage of 66.85% of the total samples. From these results it can be inferred that this series of traits and primers can detect genetic differences very well. The results of study showed that Iranian tea genotypes have a high genetic diversity since they are mostly propagated by seeds.

Keywords

Research in Horticultural Sciences
Volume 1, Issue 1
September 2022
Pages 57-72

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