Investigation on callus formation and micropropagation of ginger (Zingiber officinale Rosc.) via rhizome

Document Type : Research

Authors

1 Department of Horticultural Sciences, Faculty of Agricultural Sciences, Sari University of Agriculture and Natural Resources

2 Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resource University of Khuzestan

10.22092/rhsj.2025.361098.1046

Abstract

Ginger (Zingiber officinale Rosc.) is considered as one of the most important spices in the world, which produces spicy and fragrant rhizomes. Ginger is popular all over the globe not only as a spice but also as an herbal medicine. Propagating this plant through rhizomes is time consuming and is associated with some problems, . Due to high demand, economical and medicinal values of ginger, it seems necessary to provide a suitable protocol for mass production of ginger through tissue culture technique. Therefore, the present study investigated different hormonal compounds on rhizome buds for callus formation, induction of bud growth, rooting and adaptation for optimizing culture medium in Ginger.. Four Benzyl Adenine (BA) concentrations (0, 0.04, 0.08, 0.16 mg / l) and three Naphthalene Acetic Acid (NAA) concentrations (0.02, 0.04 mg / l) were applied alone and in combination. The highest callus formation rate was obtained from bud samples treated with 0.04, 0.08 and 0.16 mg / l NAA in combination with 0.04 mg / l of BA hormone. Optimal branch propagation was obtained from MS medium containing 0.04 mg / l of both NAA and BA hormones. The maximum rooting (85%) was obtained in 1 μM (IBA indole-3-butyric acid)..
Keywords: Auxin, In vitro, Medicinal plant, Proliferation

Keywords

Research in Horticultural Sciences
Volume 3, Issue 1
September 2024
Pages 139-148

Files

Share

How to cite

Statistics